immunoassay是什么意思,immunoassay中文翻译,immunoassay发音、用法及例句
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•immunoassay
immunoassay发音
英:[ɪmjʊnəʊ'æseɪ] 美:[ˌɪmjənoʊ'æseɪ]
英: 美:
immunoassay中文意思翻译
n. 免疫测定;免疫分析
immunoassay常见例句
1 、A novel sensitive electrochemical immunoassay method was proposed based on gold nanoparticle mediated biocatalytic deposition of platinum followed by stripping voltammetric determination.───发展了一种基于纳米金介导生物沉积铂并以铂催化氢还原伏安法进行检测的高灵敏电化学免疫分析新方法。
2 、Immunoassay is a useful technique, antibody is the key of immunoassay.───免疫分析是一项应用广泛的技术,抗体是免疫分析的关键问题。
3 、An indirect competitive enzyme-linked immunoassay(ELISA) was developed using polyclonal antibody to measure the residues of sulfaquinoxaline(SQ) in food.───利用抗磺胺喹恶啉(SQ)多抗建立酶联免疫分析方法,以测定SQ在动物源食品中的残留。
4 、The purpose of this study was to explore the clinical value of the platelet antibody screening and typing in platelets transfusion by using microcolumn gel immunoassay (MGIA).───为了探讨微柱凝胶免疫分析技术在血小板输注中血小板抗体筛选和配型的临床应用价值,运用微柱凝胶免疫分析技术检测血小板抗原抗体反应,包括抗体筛检、交叉配血。
5 、Intended use This immunoassay kit allows for the specific measurement of Mouse Procalcitonin, PCT concentrations in cell culture supernates, serum and plasma.───产品概述: 预期应用 ELISA法定量测定小鼠血清、血浆或其它相关液体中降钙素原(PCT)含量。
6 、Abstract: Chemiluminescence Immunoassay (CLIA) is an efficient detection tool combined from chemiluminescent reactions and immunoassay.───文摘:化学发光免疫技术是化学发光与免疫测定结合起来的一种高效检测手段。
7 、A novel electrochemical immunoassay method based on Au nanoparticle labeled immunocom-plex enlargement used for detection of complement C_3 was proposed in this paper.───报道了一种用金标复合物放大的计时电位法测定补体C_3的电化学免疫检测方法。
8 、The bioactivity assay of PEG-TA1, Tyr-PEG-TA1 and 125 I -Tyr-PEG-TA1 were performed by an enzyme immunoassay (EIA) method.───利用酶免疫分析(EIA)方法鉴定PEG-TA1增加酪氨酸残基前后以及Tyr-PEG-TA1经125I标记前后免疫学活性的变化。
9 、The incretion secretion metrical technique is the important embranchment in clinical diagnosis presently,in which the magnetic separate enzyme immunoassay is the main technique.───内分泌激素测量技术是医疗诊断技术发展的重要分支,磁分离酶联免疫技术是其中的主要测量方法。
10 、Methods The serum level of testosterone and estradiol were measured with radio immunoassay in 47men with rosacea.25healthy subjects were selected as control.───方法 采用放射免疫法对47例男性酒渣鼻患者血清睾酮、雌二醇进行了测定,并以25例正常男性作为对照。
11 、Moreover, We assay ADM on serum of maternal peripheral blood using enzyme immunoassay(EIA).───另外,用酶免疫测定法(EIA)测定以上各组妇女外周静脉血ADM浓度。
12 、Chemiluminescence Immunoassay (CLIA) is an efficient detection tool combined from chemiluminescent reactions and immunoassay.───化学发光免疫技术是化学发光与免疫测定结合起来的一种高效检测手段。
13 、A number of techniques and methods have been developed for the detection of DNA adducts, such as 32P-postlabelling, immunoassay, immuno-CE/LIF, LC/MS, micro-scale separation coupled MS.───当前DNA加合物分析、检测的方法主要包括:32P后标记法、免疫分析法、免疫毛细管电泳激光诱导荧光法、液相色谱串联质谱法、微分离-质谱联用法等。
14 、AFP-L3 is currently available in SGH.Test principle This assay employs the quantitative sandwich enzyme immunoassay technique.───实验原理本试剂盒应用双抗体夹心酶标免疫分析法测定标本中AFP-L3水平。
15 、John's Wort in rats.Cyclosporin concentrations in blood samples were determined by a specific monoclonal fluorescence polarization immunoassay.───利用单株抗体萤光偏极免疫分析法定量血中环孢灵之浓度。
16 、Methods: Fluorescence polarization immunoassay(FPIA) was used to measure the blood concentrations of ciclosporin and sodium valproate.───方法:采用荧光偏振免疫分析法监测环孢素A和丙戊酸钠的血药浓度,并对监测过程进行预防性和回顾性质量控制。
17 、Submandibular tissues of rats were incubated in vitro and different concentration of FSH was added.Enzyme link immunoassay was used to measure EGF and NGF in supernatant.───体外孵育大鼠下颌下腺组织并给予不同浓度FSH,应用酶联免疫分析方法检测上清液中NGF和EGF的含量。
18 、Serum FINS, FPG, TG, TC, FFA concentrations were measured with chemi-luminescence immunoassay methods.───化学发光法测HNS、FPG、TG、TC、FFA浓度。
19 、Method: The serum concentration of oral phenytoin, carbamazepine and valproic acid were determined in 26,74 and 144 epileptic patients by fluorescence polarization immunoassay, respectively.───方法:采用荧光偏振免疫法对26例服用苯妥英钠、74例服用卡马西平、144例服用丙戊酸钠的患者血药浓度监测结果及临床疗效分析、评价。
20 、SONG Shi-ping, TANG Guo-zhong, YANG Jianzhong.Experimental research on immunoassay on solid-phase avidin for Free thyroxine[J].Journal of Clinical Laboratory Science, 2003, 21 (1): 31 - 33.───[3]宋世平,唐国忠,杨建忠.固相亲合素在游离甲状腺素免疫检测中的应用[J].临床检验杂志,2003,21(1):31-33.
21 、This paper introduces the principle and design of Chinese enzyme immunoassay instrument,and emphatically discusses the design of nonlinear data processing model.───文中阐述了国产磁分离酶免疫测定仪的仪器原理及设计,对数据处理模型的设计进行了重点讨论。
22 、METHODS:Bacteria in the dental plaque of the anterior deciduous teeth of children were isolated and identified by colony morphology, biochemical test and immunoassay of serum agglutination.───方法:采用细菌分离、培养、生化试验和免疫学血清凝集试验对幼儿乳前牙牙菌斑内细菌进行分离鉴定,分析病原菌的定殖情况以及与婴儿期喂养方式的关系。
23 、The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).───应用反向间接血凝试验(RPHA)、酶免疫测定(EIA)和聚合酶链反应(PCR)测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
24 、Methods The serum E 2 levels were measured by the microparticle enzyme immunoassay (MEIA) in 26 infertile women before and after receiving induced ovulation therapy.───方法 应用微粒子酶免 (MEIA)法检测 2 6例不孕妇女接受超促排卵治疗前、后的血清E2 水平 ;
25 、In this article, we describe methodologies needed inmanufacturing the immunoassay kit from the preparationof each component to the stabilization of thesystem constructed.───在这篇文章中,我们描述了制造免疫检测试剂盒所需的方法学,从各种成分的准备到构造成的系统的稳定。
26 、Methods Serum ACA in 38 patients with DM were examined by enzyme-linked immunoassay(ELISA).We followed the trail of 17 patients with pos itive ACA.───方法应用酶联免疫吸附法(ELISA)检测了38例老年DM患者血清ACA,并对ACA**的17例患者进行了随访。
27 、Methods The quantities and activity of the TFPI were measured in 38 women with ovarian cancer by using an quantitative enzyme linked sandwich immunoassay(ELISA) and Substrate chromatography.───方法:应用双抗体夹心酶联免疫吸附法及发色底物法对38例卵巢癌患者血浆TFPI抗原含量及活性进行测定。
28 、The concentrations of follicle-stimulating hormone(FSH) , luteinizing hormone(LH) , estradiol(E2) and progesterone(P4) were tested by chemical luminescent immunoassay(CLIA).───小白泡(SW)直径为1-2mm。 血样采用化学发光法测定血浆中促卵泡素(FSH)、促黄体素(LH)、雌二醇(E_2)和孕酮(P_4)含量。
29 、Methods The serum concentration of valproic acid of 48 Petit mal children were determined by fluorescence polarization immunoassay(FPLA) .───方法 对48例癫痫失神性发作儿童患者服用丙戊酸,用荧光偏振免疫法(FPIA)监测血药浓度.
30 、Methods:Serum IGF-1 concentration in 53 patients with essential hypertension and 16 healthy persons was measured by enzyme immunoassay (EIA).───方法 :采取酶免疫分析法测定了 5 3例原发性高血压患者 (原发性高血压组 )和 16例正常人 (正常对照组 )的血清IGF 1水平。
31 、The development of enzyme immunoassays for the determination of pyrethroid residues were reviewed,including hapten design,antibody preparation,and immunoassay establishment and applications.───对拟除虫菊酯类单一农药残留和多残留酶免疫分析方法的研究进展进行了综述。
32 、Intended use This immunoassay kit allows for the specific measurement of Rat Thromboxane B2(TXB2)concentrations in serum and plasma.───ELISA法定量测定兔血清、血浆或其它相关液体中血栓素B2 (TXB2)含量。
33 、Methods Plasma HCMV-IgM antibody was detected with enzyme immunoassay (EIA) .───方法 运用酶免疫测定法(EIA)检测职业献血员中抗-HCMVIgM。
34 、CLIA,according to the labels,can be divided as Luminescent Immunoassay (LIA),Lumminescent Enzyme Immunoassay (LEIA) and Luminescent Cofactor Immunoassay (LCIA).───化学发光免疫技术可根据抗原抗体标记物的不同而分为发光物免疫测定、发光酶免疫测定和发光辅助因子免疫测定三大类型。
35 、Methods The FK506 concentration was detected by micro-particle enzyme immunoassay(MEIA),blood glucose and serum lipid was determined by automatic biochemical system CX5.───方法微粒子酶免疫法(MEIA)测定FK506血药浓度;酶法测定血糖、血脂,动态观察血糖、血脂与不同血药浓度的关系。
36 、All 26 uninfected patients had a nonreactive serum enzyme-linked immunoassay.───所有的26人的血清酶联免疫吸附试验都为阴性。
37 、Telomerase activity was determined by the telomeric repeat amplification protocol enzyme-linked immunoassay (TRAP -ELISA).───应用TRAP-ELISA法测定肾上腺肿瘤中的端粒酶活性。
38 、The immunoassay of a radiolabeled substance,such as a hormone or an enzyme.───放射免疫测定用经放射性物质标记的物质(如荷尔蒙或酶)进行免疫测定
39 、Plasma CNP level was also monitored by the enzyme immunoassay (EIA) at 1 hour, 4h ours, 1 day, 2 day, 3 day and 6 days post-injury.───并同时采用酶免疫分析法(EIA),观测伤后多时点的体内血浆CNP水平。
40 、Intended use This immunoassay kit allows for the specific measurement of human surfactant protein A (SP-A) concentrations in BALF, serum, and plasma.───产品概述: 预期应用 ELISA法定量测定人血清、血浆、肺泡灌洗液,细胞培养物上清或其它相关液体中SP-A含量。
41 、The peripheral serum FSH & LH levels were estimated by invtrion bioassay and E2 & T by immunoassay.───对E_2峰的上升速度的影响则以体穴组最大,体穴加耳穴组其次。
42 、Methods thyroid stimulating hormone (TSH) value was detected with time resolved fluorescence immunoassay (TRFIA).───方法应用先进的时间分辨荧光免疫法 TRFIA检测新生儿促甲状腺素的浓度筛查 CH。
43 、The LCR meter instrument, a new detection method for immunoassay, is used to detect the electric single.───在本研究中使用三明治型免疫分析法(三层式)使用抗体检测待测抗原,并对抗原作定量分析。
44 、Methods We useed model of swimming exhausition of rats and adopting immunoassay eazyme magnetic analysis(IEMA) method to measure the content of insulin ,C P, to test content of blood glucose and FFA in colorimetric analysis method.───方法 采用大鼠游泳力竭模型,分别用磁性微粒分离的免疫酶联测定法(IEMA)测定血清胰岛素、C肽,用比色法测定FFA、血糖,并观察力竭时间和心率的改变。
45 、A voltammetric enzyme - linked immunoassay based on a newsystem of o - aminophenol (OAP) - H2O2 - horseradish peroxidase (HRP) hasbeen used in the detection of HRP and labelled HRP.───提出了邻氨基酚(OAP)-H2O2-辣根过氧化物酶(HRP)伏安酶联免疫分析新体系并用于测定HRP及HRP标注物。
46 、Scanning Detection System of UPT-Based Immunoassay Test Strip[J].───引用该论文 卢健,周蕾,赵永凯,黄立华,刘蕾,杨瑞馥,黄惠杰.
47 、In this paper, application of immunoassay technologies in detection of food contaminants such as pesticide residues, veterinary drug residue will be briefly reviewed.───免疫分析在食品安全检测中对农/兽药残留检测有着广泛的应用前景,对几种主要的免疫分析技术进行了综述。
48 、Methods Using an quantitative enzyme linked sandwich immunoassay(ELISA)and substrate chromatography to measure the quantities and activity of the TFPI of 19 woman with carcinoma of endometrium.───方法应用双抗体夹心酶联免疫吸附法及发色底物法对19例子宫内膜癌患者血浆TFPI抗原含量及活性进行测定。
49 、Collecting venous blood of pre-anesthesia(T0),1 hour(T1),3 hours(T2) after intubation and 4 hours(T3) after operation respectively,and the content of ET was then measured via radio immunoassay.───分别于**诱导前、全麻**管后1h、3h、术后4h采静脉血,用放射免疫分析法测定内皮素含量。
50 、Utilizing the technique of immunoassay to detect the expression of transgenic plant is simple, sensitive, fast and credible.───利用免疫检测技术进行植物转基因的表达检测是一种简单、灵敏、快速、可靠的方法,但其前提条件是要有与转基因植物目的基因表达的蛋白质发生特异性免疫反应的抗体或抗血清。
51 、METHODS The SDC were determined by the fluorescence polarization immunoassay (FPIA)with TDx.───方法 采用荧光偏振免疫法 ( FPIA)测定 SDC。
52 、Soy isoflavone variety of detection methods,such as UV spectrophotometry,chromatography,chromatography and immunoassay method.───大豆异黄酮的检测方法包括紫外分光光度法、色谱法、层析法和免疫检测法等。
53 、Hapten and antiserum for parathion immunoassay───对硫**半抗原改造及其免疫抗体
54 、Fluorescence polarization immunoassay (FPIA) is comprehensively reviewed with 45 references.───对荧光偏振免疫分析技术的研究进展作了详细的评述。
55 、It's a combined optical biosensor and lateral flow immunoassay that uses both reflective surface pla*on resonance and antibodyantigen binding.─── 这个结合了光生物传感器和侧流免疫层析法 用到了反射面 等离子共振和抗体抗原结合
56 、Methods 10 kinds of bone marker were determined by enzyme immunoassay and electrochemiluminescence immunoassay in serum and urine of 164 healthy controls and 120 patients.───方法应用酶联免疫测定法或电化学发光免疫法分别测定164名健康成年人和120例患者血清和尿液中10项骨代谢指标。
57 、Legionella antigen in urine was detected using enzyme immunoassay (EIA) method.───应用酶免疫测定 (EIA)方法检测尿中嗜肺军团菌抗原。
58 、Fluorescence polarization immunoassay was used to determine the blood concentration of cyclosporin.───利用萤光偏极免疫分析法检测血中环孢灵浓度。
59 、Underlying problems in the application of immunoassay are highlighted and future trends of immunoassay development postdated.───探讨了免疫分析技术存在的问题和免疫分析的未来发展趋势。
60 、Immunoassay techniques, which also use an antibody-capture methodology, are approaching the requisite speed but lack sensitivity: a sample containing at least several thousand copies of the organism is needed to identify an agent.───免疫测定法也利用了抗体结合原理,速度接近要求,但灵敏度差:为了鉴定生物制剂,样本至少需要包含上千份有机体。
61 、The level of cAMP was measured by immunoassay KIT.───并用酶联免疫法测定细胞内环**腺苷(cAMP)的浓度。
62 、METHODS: Serum IGF-1 concentrations in 53 patients with essentia l hypertension and 16 healthy persons were measured by enzyme immunoassay (EIA).───方法:采取酶免疫分析方法测定5 3例为原发性高血压患者和16例正常人的血清IGF - 1水平。
63 、Methods Thyrotropin(TSH) concentration in dried-blood spot specimens on filter paper was dectected by enzyme immunoassay.───方法标本采集用干滤纸血片法,采用酶免法检测血中促甲状腺素浓度。
64 、Intended use This immunoassay kit allows for the specific measurement of human cytokeratin 19 (CK-19) concentrations in cell culture supernates, serum, and plasma.───产品概述: 预期应用 ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中CK-19含量。
65 、Methods:The level of Epos were checked by means of radio immunoassay in 38 cases of acute leukemia, 19 cases of deadly solid tumor and 15 cases of healthy children.───方法 :用放射免疫分析法测定 38例急性白血病 ( AL)、1 9例恶性实体瘤患儿及 1 5例健康儿童血清 Epo水平。
66 、An indirect competitive enzyme immunoassay with monoclonal antibody was established for measuring clenbuterol in urine or food.───在间接竞争酶联免疫吸附法的基础上,研制了一种可以检测尿样或食品中盐酸克仑特罗的试剂盒。
67 、New Frontiers in Agrochemical Immunoassay───农业化学制品免疫测定的新前沿
68 、A kind of chemiluminescnet immunoassay instrument is introduced in the paper.The serial communication protocol and the communication program are described in detail.───介绍了化学发光免疫定量测量仪的组成,重点阐述虚拟面板的设计、上下位机通信协议的制定和通信程序的设计。
69 、The immunoassay of a radiolabeled substance, such as a hormone or an enzyme.───放射免疫测定用经放射性物质标记的物质(如荷尔蒙或酶)进行免疫测定
70 、The accuracy of time-resolved fluorescence immunoassay (TRFIA) instrument depends on the anti-noise capacity of the measurement circuit for weak fluorescence signals.───摘要时间分辨荧光免疫分析仪的测量精度取决于其对弱荧光信号检测电路的抗噪声能力。
71 、Enzyme-Linked Immunosorbent Assay (ELISA) is an immunoassay technology established on the combination of the highly specific antigen-antibody reaction and the efficient catalysis of enzyme.───摘要酶联免疫吸附是将抗原抗体反应的高度特异性和酶的高效催化作用相结合发展建立的一种免疫分析技术。
72 、Methods FT3, FT4 were determined by immunoassay system in 71 patients with hyperthyroidism and 32 healthy controls.TG, TCH, HDL-C and total protein were determined at the same time.───方法用免疫化学发光技术检测71例患者和32例健康对照组血清游离T3(FT3)、游离T4(FT4),以生化仪检测甘油三酯(TG)、胆固醇(TCH)、高密度脂蛋白胆固醇(HDL-C)和总蛋白的含量。
73 、Methods Rats used in this experiment were injected immunosuppressant into their abdomen.The immunoassay was made after acupuncture and irradiation at Baihui point by semiconductor laser.───方法实验中大鼠腹腔注射免疫抑制剂后,分别用针刺和激光疗法进行治疗,然后测定大鼠NK细胞的活性。
74 、Immunoassay is the traditional method for analysis of substances in trace amounts,combining the specificity of immunoreactions with sensitivity of the tracer.───免疫分析是利用免疫反应的特异性和示踪物的灵敏性进行物质超微量分析的传统方法。
75 、The incretion secretion metrical technique is the important embranchment in clinical diagnosis presently, in which the magnetic separate enzyme immunoassay is the main technique.───摘要内分泌激素测量技术是医疗诊断技术发展的重要分支,磁分离酶联免疫技术是其中的主要测量方法。
76 、Because the signal-to-noise ratio is an essential indicator of system performance, there should be an optimal concentration of antibody for a given immunoassay system.───因为信号-噪声比例是系统性能的本质指示,所以对一个给定的免疫检测系统来说存在一个最佳的抗体浓度。
77 、Method:The levels of cytokine and immunoglobulin in sera of 23 patiants with RP were measured by ELISA and immunoassay and were compared with the normal data published by our department of Laboratory.───方法:对23例RP患儿行外周血细胞因子及免疫球蛋白水平的检测,并与我院检验科测定的正常值进行比较。
78 、The chemiluminescence analysis and the chemiluminescence immunoassay (CLIA) are a kind of analysis technology of ultromicro biomatters.───化学发光分析法和化学发光免疫分析法是近期发展起来一项超微量生物活性物质分析技术。
79 、Methods:The CEA,CA125,CYFRA21-1 contents of 71 cases of lung cancer,20 cases of benign lung disease and 20 cases of healthy controllers were detected by electrochemiluminescence immunoassay(ECLIA).───方法:采用电化学发光法分别检测71例肺癌患者和20例肺良性疾病患者及20例健康者的血清中CEA、CA125、CYFRA21-1的含量。
80 、Methods: We examined and observed 52 patients with typhoid and 20 normal controls by bacterial culture, Widal reaction, and enzyme-linked immunoassay for detection of typhoid bacilli H antigen.───方法该文采用细菌培养、肥达反应、伤寒杆菌H抗原酶联免疫检测检查52例伤寒患者,20例正常人,进行对照观察。
81 、There are three kinds of biosensors relevant to bioluminescence &chemiluminescence: immobilized enzyme sensor, immunoassay sensor and report gene sensor with specialized regulator.───基于生物及化学发光的生物传感器主要有三类 :酶固化传感器、免疫分子传感器和特异调控因子诱导的报告基因传感器。
黄曲霉的检验检疫方法是怎样的?
答: 薄层层析法 薄层层析(thin-layer chromatography,tlc)是在黄曲霉毒素研究方面应用最广的分离技术.自1990年,它被列为aoac (association of official agricultural chemists)标准方法,该方法同时具有定性和定量分析黄曲霉毒素的功能. 液相色谱法 液相色谱(liquid chromatography,lc)与薄层层析在许多方面具有相似性,二者互相补充.通常用tlc进行前期的条件设定,选择适宜的分离条件后,再用lc进行黄曲霉毒素的定量测定. 免疫化学分析方法 利用具有高度专一性的单克隆抗体或多克隆抗体设计的黄曲霉毒素的免疫分析方法,也是最常用的黄曲霉毒素检测方法.这类方法通常包括放射免疫分析方法(radioimmunoassay,ria),酶联免疫法(enzyme-linked of immunosorbent assay,elisa)和免疫层析法(immunoaflinity column assay,ica).它们均可以对黄曲霉毒素进行定量测定. (1) 免疫亲和柱-荧光分光光度法和免疫亲和术-hplc法 免疫亲和柱法和酶联免疫吸附法虽然都可达到速简便效果,但酶联免疫吸附法仅能检测单一毒素(如黄曲霉毒素b1)含量,而且易出现假**结果,难以控制.免疫亲和柱法(包括荧光光度法和hplc法)却能达到既定量准确又快速简便的要求. 免疫亲和柱的使用可以避免传统tlc和hplc的缺点,同时免疫亲和柱与tlc和hplc法结合可以大大提高工作效率,提高灵敏度和准确度. 黄曲霉毒素免疫亲和柱-荧光光度计法是以单克隆免疫亲和柱为分离手段,用荧光计,紫外灯作为检测工具的快速分析方法.它克服了tlc和hplc法在操作过程中使用剧毒的 真菌毒素作为标定标准物和在样品预处理过程中使用多种有毒,异味的有机溶剂,毒害操作人员和污染环境的缺点.同时黄曲霉毒素免疫亲和柱-荧光光度计法分析速度快,一个样品只需10-15min,比传统方法快几个小时甚至几天时间;仪器设备轻便容易携带,自动化程度高,操作简单,直接读出测试结果,可以在小型实验或现场使用.可以进行黄曲霉毒素总量 (b1b2g1g2) 的测定,检测限可达到1ug/kg,达到黄曲霉毒素标准限量值以下测定范围为1-300ug/kg. 黄曲霉毒素免疫亲和柱-高效液相色谱法比传统的hplc法更加安全,可靠,灵敏度和准确度高.它采用单克隆抗体免疫技术,可以特效性地将黄曲霉毒素或其他真菌毒素分离出来,分离效率和回收率高. 分析原理试样中的黄曲霉毒素用一定比例的甲醇/水提取液经过过滤,稀释后,用免疫亲和柱净化,以甲醇将亲和柱上的黄曲霉毒素淋洗下来,在淋洗液中加入溴溶液衍生,以提高测定灵敏度,然后用 荧光分光光度计进行定量.也可以将甲醇-黄曲霉毒素淋洗液的一部分注入hplc中,对黄曲霉毒素b1,b2,g1,b2分别进行定量分析.免疫亲和柱是用大剂量的黄曲霉毒素单克隆抗体固化在水不溶性的载体上,然后装柱而成.该方法的测定范围0-300ug/kg. (2) 酶联免疫吸附法: 1996年,nakane 建立了 辣根过氧化物酶标记抗体的测定技术.由于该方法简便,敏感,特异,可作为多种抗原或抗体的测定,20世纪70年代后期,该方法引入真菌毒素的检测中,下面介绍的是竞争性酶联免疫吸附间接法检测黄曲霉毒素b1. 原理:将已知抗原吸附在固态载体表面,洗除末吸附抗原,加入一定量抗体与待测样品(含有抗原)提取液的混合液,竞争培养后,在固相载体表面形成抗原抗体复合物.洗除多余抗体成分,然后加入酶标记的抗球蛋白的第二抗体结合物,与吸附在固体表面的抗原抗体结合物相结合,再加入酶底物.在酶的催化作用下,底物发生降解反应,产生有色物质,通过酶标检测仪测出酶底物的降解量,从而推知被测样品中的抗原量. (3) 微柱筛选法 可以用来半定量测定各种食品中黄曲霉毒素b1,b2,g1,g2的总量. 原理 样品提取液中的黄曲霉毒素被微柱管风硅镁型吸附层吸附后,在波长365nm紫外光灯下显示蓝紫色荧光环,其荧光强度与黄曲霉毒素在一定的光密度范围内 成正比例关系.若硅镁型吸附剂层未出现蓝紫色荧光,则样品为阴性(方法灵敏度为5-10ug/kg).由于在微柱上不能分离黄曲霉毒素b1,b2,g1,g2,所以测得结果为总的黄曲霉毒素含量. (4) 一步式黄曲霉毒素检测 金标试纸法 一步式黄曲霉毒素检测金标试纸法是利用单克隆抗体而设计的固相免疫分析法.由此产生的一步式黄曲霉毒素快速检测试纸可在5—10分钟完成对样品中黄曲霉毒素的定性测定.借助黄曲霉毒素标准样品,这种方法能估算黄曲霉毒素的含量,非常适用于现场测试和进行大量样品的初选.
hr医学上是什么意思
HR医学上是心率的缩写,也是一个非常重要的医学指标。通过测量心率,医生可以判断一个人的心脏健康状况以及身体的代谢状态。正常人的心率一般在60-100次/分钟之间,如果超过或低于这个范围,可能代表心脏存在问题或身体系统出现异常状况。
HRV医学上是心率变异性的缩写,用来描述心率的变化规律。通过分析连续的心率数据,医生可以确定一个人是否处于紧张、放松或者休息状态。荧光素酶联免疫分析(FIA,Fluorescence Immunoassay)是HRV的常用检测方法。HRV可以反映一个人的自主神经功能,是一个衡量身体是否处于最佳状态的重要指标。
HR也是一个企业中经常被提到的标准缩写,代表Human Resources,中文翻译为人力资源。HR部门通常是一支专门负责企业管理、招聘、培训等方面的团队,是企业成功的关键之一。在企业管理中,HR的作用不可忽视,能为企业提供合适的人才,优化组织结构和管理流程,提高企业的效率和竞争力。